Lian-Jia-San-Jie formula suppresses non-small cell lung cancer via FTO-dependent m6A demethylation regulation of lncRNA CASC9 and its downstream CD3EAP

Background

Dysregulation of the epitranscriptome is implicated in the aberrant expression of oncogenes during oncogenesis. In this study, we sought to elucidate the therapeutic mechanisms of Lian-Jia-San-Jie-Fang (LJSJF), a traditional Chinese medicinal formula composed of 14 herbs with Scutellaria barbata D. Don (whole herb) as the principal component, together with Sarcandra glabra (Thunb.) Nakai (whole herb), Ranunculus ternatus Thunb. (whole herb), Adenophora tetraphylla (Thunb.) Fisch. (root), Glehnia littoralis F.Schmidt ex Miq. (root), Pseudostellaria heterophylla (Miq.) Pax (tuberous root), Ophiopogon japonicus (Linn. f.) Ker Gawl. (root), Asparagus cochinchinensis (Lour.) Merr. (tuberous root), Rubia cordifolia L. (root and rhizome), Pinellia pedatisecta Schott (tuber), Hedyotis diffusa Willd. (whole herb), Bombyx batryticatus (dried larva), and Inula japonica Thunb. (flower) at a fixed clinical mass ratio, prepared by water extraction, concentration, and lyophilization.