Cancer management and research
Development of a Multiplex PCR to Detect the BCR-ABL1 Transcripts in Chronic Myeloid Leukaemia Patients in Indonesia
By Prihatni D, Nafsi NI, Ernawati E and 3 others.
Abstract
Background
There are limited commercial kits available for the identification of BCR-ABL1 gene fusion transcripts in chronic myeloid leukaemia (CML) patients. Most kits are based on RT-PCR to detect e14a2 and e13a2 transcripts as major breakpoint segments, but do not detect minor transcripts. Therefore, this study aimed to develop a multiplex PCR approach for BCR-ABL gene transcripts.