Employing Streptomyces enissocaesilis as a sustainable cell factory for green synthesis of gold nanoparticles with multi-target anticancer activity against MCF-7 breast cancer cells

Background

Currently, there is growing interest in gold nanoparticles (AuNPs) to be employed in cancer nanomedicine. This study describes the green synthesis of AuNPs using an actinobacterial isolate identified as Streptomyces enissocaesilis via 16 S rRNA sequencing. Characterization of the nanoparticles was performed using various techniques including UV-Vis spectroscopy, FTIR, TEM, XRD, EDX, and zeta-potential analysis. To provide insight into the interaction of AuNPs with MCF-7 breast cancer cells, a variety of apoptosis and cell proliferation assays were performed. These assays included concentration-dependent cytotoxicity testing, cell-cycle analysis, an Annexin V-FITC/PI apoptosis analysis, AO/PI staining, a BrdU proliferation assay, and an RT-qPCR to evaluate the expression of c-erbB-2 and p53.