Gene and Isoform Misassignment Among Candidate TP53 and CDKN2A Variants of Uncertain Significance in Oral Squamous Cell Carcinoma: A Public-Database Curation Study

Background

Variants of uncertain significance (VUSs) in TP53 and CDKN2A are reported often in oral squamous cell carcinoma (OSCC), yet variant-level evidence tends to lag behind established gene-level associations. Candidate variant lists compiled by automated literature synthesis, registry mining, or AI-assisted extraction carry a risk of gene-level or transcript-level mis-annotation that is not widely recognized.