Journal of Radiation Research and Applied Sciences
ANP32B knockdown suppresses glioma malignant progression via the E2F1 pathway
By Xiaomei Wan, Lilong Wu, Chengcheng Huang and 5 others.
Abstract
Background
Glioma is an aggressive primary brain malignancy with a poor prognosis. This study aimed to investigate the mechanism by which ANP32B drives glioma progression, focusing on its regulation of E2F1-mediated cell cycle and malignant phenotypes.
Methods
Bioinformatics analysis was first performed to verify the differential expression of ANP32B mRNA in glioma tissues and normal brain tissues. A series of in vitro functional experiments including CCK-8 assay, colony formation assay and wound healing assay were carried out to evaluate the changes in proliferation, colony formation and migration capabilities of LN229 and U87MG glioma cell lines after ANP32B knockdown. Meanwhile, in vivo tumorigenesis experiments were conducted to confirm the regulatory effect of ANP32B downregulation on glioma growth. In addition, RNA sequencing technology combined with functional rescue experiments was applied to screen, analyze, and validate the downstream signaling pathways mediated by ANP32B in glioma cells.