Spatially Resolved Transcriptomics in Tumor Organoids: Unraveling Immune Microenvironment Heterogeneity in 3D

Background

Tumor organoids recapitulate the three-dimensional architecture, cellular composition, and immune microenvironment of the originating tumor, offering a reliable in vitro platform for immuno-oncology research. However, understanding the spatial organization of tumor–immune interactions within these models requires technologies that preserve tissue context.