Frontiers in microbiology
A multi-matrix workflow for isolation, characterization, and biobanking of antimicrobial- resistant bacteria from One Health surveillance samples
By Bahr A, Heiden SE, Karnatz D and 7 others.
Abstract
Objectives
Standardized protocols for the isolation of antimicrobial-resistant (AMR) bacteria are typically optimized for individual sample types, limiting comparability across One Health surveillance domains. Here, we describe a multi-matrix workflow for the isolation, characterization, and biobanking of AMR bacteria (Enterobacterales, Pseudomonadales and Staphylococcus aureus) from diverse One Health sample types, with the aim of enabling reproducible and comparable AMR surveillance across sectors and settings.