Frontiers in allergy
The optimized endolysosomal degradation assay with standardized enzyme activity represents an orthogonal method for predicting protein immunogenicity and T cell epitopes
By Öztemiz E, Regl C, Brandstetter H and 3 others.
Abstract
Introduction
The endolysosomal degradation assay mimics endolysosomal processing in antigen-presenting cells in vitro and can be used to evaluate protein immunogenicity and T cell epitopes. As previous endolysosomal degradation results demonstrated significant variability, we developed an optimized protocol to ensure the reproducibility and reliability of the assay.
