Alcalase-hydrolyzed casein peptides after simulated gastrointestinal digestion alleviate high-fat diet-induced metabolic dysfunction in mice

Background and aim: High-fat diet (HFD)-induced metabolic dysfunction is associated with disturbances in gut microbial composition and hepatic lipid metabolism. This study investigated the metabolic effects of Alcalase-hydrolyzed casein peptides after simulated gastrointestinal digestion (MCPs) in HFD-fed mice and explored their potential association with gut microbiota and fecal metabolic alterations.