Gastroenterology and hepatology from bed to bench.
Stepwise purification, characterization, and diagnostic evaluation of antigen B isolated from Echinococcus granulosus hydatid cyst fluid for indirect ELISA detection of human cystic echinococcosis: Echinococcus granulosus hydatid cyst fluid for indirect ELISA detection of human cystic echinococcosis
By Mona Maleki, Sara Nemati, Farzaneh Hosseini and 2 others.
Abstract
Background
Cystic echinococcosis (CE) is a globally significant zoonosis requiring improved, cost-effective serodiagnosis. Accurate detection is often hindered by cross-reactive host contaminants in crude hydatid cyst fluid (HCF). This study aimed to purify and evaluate the immunogenicity of native Antigen B (AgB) extracted from sheep HCF for indirect ELISA detection of human CE.