Comparative Antisickling Activity and Pharmacognostic Characterisation of Spondias mombin Leaf and Stem Bark

Aims

This study aims to comparatively evaluate the antisickling activity and pharmacognostic characteristics of the leaves and stem bark of Spondias mombin, with a view to providing scientific evidence for their potential use in sickle cell disease management and establishing diagnostic parameters for their identification and standardisation. Place and Duration of Study: Drug Research and Production Unit, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria, between October 2024 and September 2025. Methodology: Dried plant materials were extracted with 70% ethanol, freeze-dried, and reconstituted in phosphate-buffered saline. Inhibitory and reversal antisickling assays were performed on HbSS erythrocytes at concentrations of 2 and 4 mg/mL using sodium metabisulphite-induced sickling. Cytotoxicity was assessed by percentage haemolysis and methaemoglobin (MetHb) formation, while pharmacognostic authentication was performed using standard anatomical procedures.