An Exploratory Study of the Potential Antibiotic-Enhancing Effect of Bee Venom Against Uropathogens in Patients with Type 2 Diabetes Mellitus at Brack General Hospital, Libya

Antimicrobial resistance is a growing global health concern, necessitating alternative strategies to improve antibiotic efficacy. This study aimed to conduct an in vitro exploratory assessment of the potential antibiotic-enhancing effect of bee venom against uropathogens isolated from patients with type 2 diabetes mellitus at Brack Al-Shati General Hospital, Libya. The study focused on observing preliminary changes in antibiotic susceptibility in the presence of bee venom rather than quantitatively evaluating antibiotic synergy. Materials and Methods: This laboratory-based exploratory study included 51 patients with T2DM (29 males and 22 females), aged ≥40 years, recruited from July 20 to October 1, 2023. Urine samples underwent urinalysis and bacterial culture, followed by isolate identification using conventional methods and the API 20E system. Although 51 patients were enrolled, only nine bacterial isolates were recovered and included in the antimicrobial experiments. Antibiotic susceptibility was assessed using the Kirby–Bauer disk diffusion method, and results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) M100-S27, 27th edition (2017), using species- and antibiotic-specific interpretive criteria. A volume of 50 µL of undiluted bee venom preparation was applied to each antibiotic disk, and changes in inhibition-zone diameters were assessed descriptively. The Wilcoxon signed-rank test was used for paired comparisons, with emphasis on effect sizes given the small number of isolates. Quantitative synergy assays were not performed.