Viruses
Application Value of In-House HIV-1 DNA Genotypic Resistance Testing for Individuals with Low-Level Viremia
By Ying Zhou, Chuanfeng Zhang, Qi Sun and 7 others.
Abstract
Background
To provide evidence for HIV-1 DNA genotypic resistance testing (GRT) strategies in low-level viremia (LLV) populations by comparing the amplification efficiency and resistance mutation profiles between HIV-1 DNA GRT and RNA GRT. (2)
Methods
Convenience sampling was used for participant enrolment. Plasma HIV-1 RNA and peripheral blood monocyte (PBMC) DNA were extracted separately. The protease–reverse transcriptase (PR-RT) region was amplified via an in-house protocol, followed by Sanger and Nanopore sequencing. The exact McNemar’s test and Fisher’s test were applied to compare amplification success rates. Univariate and multivariable logistic regression analyses implemented in R were conducted to identify factors associated with the detection of HIV-1 drug-resistant strains. BEAST-based molecular clock analysis was performed to calculate the divergence time between RNA-derived and DNA-derived sequences. (3)