Assay Performance and Quantification Limits in Perioperative Serum Cytokine Measurement After Total Hip Arthroplasty: A Secondary Laboratory Analysis

Background

Commercial cytokine assays may not span the concentrations encountered in perioperative serum. We examined the extent to which reconstructed enzyme-linked immunosorbent assay data from a total hip arthroplasty cohort could support temporal biological interpretation when most IL-6 and IL-10 results were below the validated quantification range.