Phylogenetic Insights into

Hydatid disease caused by the Echinococcus pyogenes parasite is one of the most dangerous zoonotic diseases in humans and animals, with a wide spread. It is considered a fatal disease in cases of severe infection in the brain or heart in humans, in addition to the economic loss in the field of livestock. The research included identifying the genetic patterns of the hydatid parasite in humans and animals in some areas of Iraq using the COX1 and NAD1 genes. Binary alignment operations using NCBI-Blastn based on the COX1 gene revealed the presence of four nucleic acid variations (92T>C, 102C>T, 147C>T, and 293T>C) in some samples.The parasitic samples carrying the COX1 gene are the primary ancestors from which the other genotypes arose. Analysis of the NAD1 subunit using NCBI-Blastn in the parasitic samples revealed two types of nucleic acid alterations (129A>G, 279T>C, and 558A>G) in some samples. All studied samples, whether isolated from humans or sheep, fall within genotype 1 in the Echinococcus granulosus sequences. Due to the high nucleic acid diversity, sample H10 exhibited the highest frequency of the aberration compared to other sequences of the same genotype. Preliminary sequences submitted to NCBI for GenBank OR150458- OR150471 (for COX gene), Preliminary sequences submitted to NCBI for GenBank OR166776- OR166779 (for NAD1 gene), Echinococcus granulosus strain ANASOHI isolate.