Methods in molecular biology (Clifton, N.J.)
A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3)
Abstract
Continuous processing allows to maximize space-time yields and is implemented in many industrial branches. However, in manufacturing of value added compounds produced with microbial hosts, continuous processing is not state-of-the-art yet. This is because fluctuating productivity causes unwanted process deviations. Cascaded continuous bioprocessing, unlike conventional continuous process modes, was found to result in stable productivity. This manuscript serves as a guideline how to set up a cascaded continuous cultivation with Escherichia coli BL21 DE(3).
Source and rights
Published by Methods in molecular biology (Clifton, N.J.), 2022. The guideline itself is at the source linked here.
Prognia summarises this guideline for reference and education. It is not medical advice. Always check the official guideline and current local protocols before acting on it.
At a glance
- Published
- 2022
- Applies to
- 🌐 International
- Country of origin
- 🇦🇹 Austria
- Scope
- International
- Journal
- Methods in molecular biology (Clifton, N.J.)
- PubMed
- 34519978
- Added to Prognia
- 22 July 2026