Frontiers in immunology
CircUGP2 inhibits tumor progression via PI3K/AKT/mTOR signaling through sponging miR-137-3p and interacting with UPF1 in hepatocellular carcinoma
Por Xie L, Li S, Guo H y 11 más.
Contenido en inglés
Resumen
Background
Circular RNA (circRNA) has been shown to have a pivotal role in the initiation and evolution of multiple tumors, but its function in hepatocellular carcinoma (HCC) requires further elucidation.
Methods
circUGP2 (hsa_circ_0001020) was identified in the GEO database by HCC circRNA microarray. Subsequently, an assessment was conducted to determine the biological effects of circUGP2, employing a series of analytical methods, including CCK8, EdU, colony formation, transwell, scratch assays, and in vivo experimentation. To explore the functions of circUGP2 as a sponge, including RNA pull-down, microRNA-seq, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP), and luciferase reporter assays were employed. Mass spectrometry (MS), RIP, and co-inmunoprecipitation (Co-IP) were employed to examine the interaction between cirrcUGP2 and up-frameshift protein 1 (UPF1).