Frontiers in nutrition
Lactobacillus rhamnosus and Lactobacillus gasseri ameliorate alcohol-induced liver injury and gut microbiota dysbiosis in rats
Por Zhang Y, Liu H, Fu Y y 5 más.
Contenido en inglés
Resumen
Introduction/objective
This study aimed to evaluate whether Lactobacillus rhamnosus and Lactobacillus gasseri could alleviate alcohol-induced liver damage, behavioral changes, and gut microbiota dysbiosis in rats. And sought to better understand the role of Lactobacillus-mediated microbiota modulation in alcohol-induced liver damage.
Methods
Male SD rats were first subjected to 1 week of ethanol adaptation, followed by 4 weeks of feeding with a Lindros alcohol liquid diet containing 6.2% ethanol to establish the ALD model. Subsequently, the rats received 2 weeks of either L. rhamnosus or L. gasseri (1 × 109 CFU/rat/day) treatment while continuing to receive the Lindros alcohol liquid diet containing 6.2% ethanol. The control group were treated with PBS. Serum fibroblast growth factor 21 (FGF21), lipopolysaccharide (LPS), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were determined, along with hepatic TNF-α expression. Behavioral performance was assessed using the open-field test, and gut microbiota composition was analyzed by 16S rRNA gene sequencing.