International journal of genomics
Integrative Single-Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma
Por Jiao P, Chen X, Wei H y 5 más.
Contenido en inglés
Resumen
Background
Osteosarcoma contains heterogeneous malignant-cell states whose clinical relevance is difficult to infer from small single-cell cohorts.
Methods
We reanalyzed six treatment-naive osteosarcomas from GSE162454, identified CNV-supported malignant osteoblastic cells, and characterized a recurrent proliferative/mitotic state using patient-paired pseudobulk analysis and program-selection sensitivity tests. A 50-gene module was evaluated for association with metastatic occurrence in 53 pretreatment tumors from GSE21257. Candidate ranking was audited across transparent weighting scenarios, and TROAP was depleted with two independent siRNAs in HOS and U2OS cells.