Journal of Pain Research
Mild Moxibustion Alleviates Primary Dysmenorrhea in Rats Through TRPV1-Associated Modulation of NF-κB Signaling and Uterine Oxidative Stress
Por Kuo Bi, Xiran Xiao, Bingna Hou y 7 más.
Contenido en inglés
Resumen
Purpose
To investigate the therapeutic effect and analgesic mechanism of mild moxibustion at Shenque (CV8) and Guanyuan (CV4) in a primary dysmenorrhea (PD) rat model, focusing on the involvement of TRPV1-NF-κB signaling and uterine oxidative stress.
Methods
Forty female Sprague-Dawley (SD) rats were randomly divided into five groups (n=8): blank group (BG), model group (MG), mild moxibustion group (MMG), antagonist capsazepine group (CPZG), and ibuprofen group (IG). PD models were established through cold stimulation combined with estradiol benzoate and oxytocin injection. The MMG received mild moxibustion at "Shenque” (CV8) and "Guanyuan” (CV4) acupoints. The CPZG was pretreated with capsazepine (CPZ) before moxibustion. The IG received ibuprofen via gavage. After intervention, pain thresholds and writhing scores were measured; uterine pathology was observed via HE staining; ELISA, colorimetric assay, immunohistochemistry (IHC), and Western blotting were used to detect relevant biomarkers.