Frontiers in allergy
Exploration of miRNAs promoting eosinophilia and type 2 immunity
By Sayedali H, Calvén J, Boberg E and 6 others.
Abstract
Background
MicroRNAs (miRNAs) are evolutionary conserved, small non-coding RNAs that regulate gene expression and modulate cellular responses. In this study, we profiled miRNAs regulated by interleukin-33 (IL-33), a key inflammatory mediator of allergic responses and type 2 immunity that promotes bone marrow eosinophil development and subsequent airway recruitment.
Method
C57BL/6 mice were exposed to recombinant IL-33 intranasally to induce airway eosinophilia. Cellular composition was determined by differential cell counts in bronchoalveolar lavage fluid and bone marrow. RNA profiling was performed using miRNA microarray, NanoString nCounter (miRNA and mRNA), qPCR (miRNA), and PrimeFlow-a method combining flow cytometry with single-cell miRNA detection.
