Journal of inflammation research
Integrated Single-Cell and Bulk RNA Sequencing Reveals Inflammatory Microenvironment and Glycolysis-Driven Epithelial Proliferation in Benign Prostatic Hyperplasia
By Ge J, Jiao W, Yu W and 3 others.
Abstract
Background
Benign prostatic hyperplasia (BPH) is a prevalent condition and a leading cause of urinary dysfunction in middle-aged and elderly men. The hyperplasia of prostate stromal and epithelial cells enlarges the prostate, compressing the bladder outlet and resulting in obstructive symptoms. However, the crosstalk between the inflammatory microenvironment and metabolic alterations in BPH remains poorly understood.
Methods
This study integrated single-cell RNA sequencing and spatial transcriptomics datasets to characterize the biological and metabolic properties of BPH. Analyses were conducted using CellChat, CytoTRACE, Monocle, and SCENIC, alongside functional pathway exploration. The CARD algorithm mapped single-cell clusters onto spatial transcriptomics, enabling subtype-specific investigations of cell dependencies and pathway activities. Bulk RNA sequencing of rat prostate tissues and in vivo experiments were performed to validate these findings.
