ABRAXAS1-HELQ is a candidate class-matched reference chimeric RNA and contributes to prostate cell motility through MCOLN2

Background

RT-qPCR is widely used for RNA expression quantification, and reliable normalization requires appropriate reference genes. Because different RNA classes display distinct expression characteristics, class-matched internal controls are essential for reliable quantification. While class-matched reference genes have been established for microRNAs and circular RNAs, stable reference genes for chimeric RNAs in the prostate remain lacking, limiting the accuracy of expression analyses. Recent evidence indicates that chimeric RNAs also exist under physiological conditions, suggesting that some chimeric RNAs may function as housekeeping-like molecules.