Abstract / Summary
Background: To investigate blood levels and the importance of Müllerian Inhibiting Substance (MIS), insulin-like growth factor 1 (IGF-1), insulin-like growth factor binding protein 3 (IGFBP-3), and 25-hydroxyvitamin D S25(OH)DC in patients with polycystic ovarian syndrome (PCOS).
Methods: The observation group consisted of 212 PCOS patients who received treatment at this institution between May 2022 and May 2024, while the control group consisted of 212 healthy women who were physically examined at the same hospital during the same time frame. The two groups' general information, serum 25(OH)D, MIS, IGF-1, and IGFBP-3 levels, and sex hormones and glucose metabolism markers were compared. PCOS patients were divided into the IR group and the non-IR group based on the presence of insulin resistance (IR). The levels of serum 25(OH)D, MIS, IGF-1, and IGFBP-3 were compared between the two groups. The study examined the associations among the four markers and the homeostasis model of insulin resistance index (HOMA-IR), as well as their supplementary diagnostic utility for PCOS.
Results: The level of 25(OH)D in the observation group was lower than that in the control group (P< 0.05). Total testosterone (TT), luteinising hormone (LH), estradiol (E2), fasting insulin (FINS), fasting blood glucose (FBG), serum MIS, IGF-1, IGFBP-3, HOMA-IR, and the average body mass index were all greater in the observation group than in the control group (P< 0.05). The IR group's 25(OH)D level was lower than the non-IR group's (P< 0.05). Serum MIS, IGF-1, and IGFBP-3 levels were higher in the IR group than in the non-IR group (P< 0.05). The levels of serum MIS, IGF-1 and IGFBP-3 in the observation group were positively correlated with HOMA-IR (r= 0.659, 0.586, 0.565, P< 0.05), and 25(OH)D was negatively correlated with HOMA-IR (r=-0.548, P< 0.05). The combined auxiliary diagnosis of PCOS by serum 25(OH)D, MIS, IGF-1, and IGFBP-3 had an area under the curve of 0.963, according to the results of receiver operating characteristic curve analysis, which was greater than that of the single detection of each index (0.819, 0.864, 0.814, 0.799).
Conclusions: MIS, IGF-1, IGFBP-3, and serum 25(OH)D levels are associated with the development and occurrence of PCOS. The combined detection of these four indicators has high auxiliary diagnostic value for PCOS and is conducive to clinical prevention and treatment.