Journal of pain research
Re-Analysis of Public Transcriptomes Identifies a Candidate Histone-Cluster Signal in Eutopic Endometrium: A Hypothesis-Generating Link to Chronic Pelvic Pain
By Cui X, Liu J, Wang S and 5 others.
Abstract
Background
Endometriosis is a leading cause of chronic pelvic pain (CPP), but the public transcriptomic datasets analysed here contain no pain phenotype, links to CPP are therefore hypothesis-generating.
Methods
We re-analysed GSE153739 (four endometriosis, three controls) and tested replication in GSE51981 (77 cases, 34 disease-free controls). Non-reproducible differential-expression results from the original submission were removed. Both cohorts were rebuilt from source processed matrices. Histone-cluster scores were modelled with menstrual-cycle and proliferation covariates, and ranked enrichment was tested before and after histone-gene depletion. A separate ion-channel analysis was pre-specified before execution.
