Pengaruh Lama Pendinginan Sebelum Ekuilibrasi Terhadap Kualitas Spermatozoa Sapi Simmental Pasca Ekuilibrasi

The purpose of this study was to determine the effect of pre-equilibration cooling time on the post-equilibration quality of Simmental bull spermatozoa. This research was conducted at the Laboratory of the Regional Technical Implementation Unit (UPTD) of the Livestock Breeding Center, Office of Food Crops, Horticulture, and Animal Husbandry of Jambi Province from October 10 to November 10, 2025. An experimental method was used with a completely randomized design (CRD) consisting of four treatments and six replications. The treatments included P0 (2 hours of pre-equilibration cooling), P1 (4 hours of pre-equilibration cooling), P2 (6 hours of pre-equilibration cooling), and P3 (8 hours of pre-equilibration cooling). The results showed that the pre-equilibration cooling time significantly affected the motility, viability, and membrane integrity of Simmental bull spermatozoa (P < 0.05). It can be concluded that the optimum pre-equilibration cooling time to produce good quality Simmental bull spermatozoa is 2 to 4 hours.