Abstract / Summary
PurposeAlthough chemotherapy can disrupt homeostasis of gut microbiota, the role of chemotherapy affecting in gut microbiota of pancreatic ductal adenocarcinoma (PDAC) is unclear. Materials and MethodsWe evaluated whether chemotherapy could alter the integrity of gut microbiota of PDAC-bearing mouse in vivo.Using a xenogenic orthotopic PDAC-bearing BALB/c nude mouse model by inoculation of BxPC-3-luc into the pancreas, we monitored tumor volumes and analyzed gut microbiota profiles via 16S rRNA sequencing, comparing mice systemically treated with gemcitabine/nab-paclitaxel (OC, orthotopic PDAC chemotherapy), those treated with 1× phosphate-buffered saline (ON, orthotopic PDAC non-treated), and normal controls (NC). Results Nodifferences were found in alpha diversity; however, phylogenetic diversity increased significantly in OC mice compared to ON mice.Phylogenetic analysis at the family level showed that abundances of Lachnospiraceae, Oscillospiraceae, and Bacteriodaceae were similar in OC and ON.However, Borkfalkiaceae and Lactobacillus significantly increased in OC compared to ON. Unclassified bacterial genera lacking formal taxonomic assignments and functional annotations, such as Clostridium, FMGN, PAC000663, and PAC001360, increased significantly in OC compared to ON.Further, a pathobiont of Escherichia increased significantly in ON compared to NC, but was depleted in OC. ConclusionThis is the first study to demonstrate recovery of a partial, chemotherapy-associated shift toward a A c c e p