Asian Pacific Journal of Tropical Biomedicine
Ginkgolide B restores pancreatic β-cell identity and attenuates oxidative stress in streptozotocin-induced diabetic rats via Nrf2/Keap1 pathway activation
By Najam us Sahar Khalid, Shikha Shah, Muhammad Sarwar and 4 others.
Abstract
Objective
To investigate the efficacy of ginkgolide B, a major active constituent isolated from Ginkgo biloba , on pancreatic β-cell integrity and Nrf2/Keap1 signaling in a streptozotocin (STZ)-induced diabetes model of rats.
Methods
Sprague-Dawley rats were injected with STZ along with nicotinamide. After confirmation of hyperglycemia, diabetic rats ( n =8/group) received no treatment, or ginkgolide B (10 or 30 mg/kg). Healthy rats served as normal control. After 21 days of treatment, rats were sacrificed, and plasma samples was obtained to assess the biochemical, hepatic, renal, oxidative stress, lipid profile, apoptotic, and inflammatory parameters. Pancreatic tissue was used for histopathological observation and detection of gene expression of NFE2L2, KEAP1, NQO1, HO-1, Ins1, MAFA, Ngn3, Pdx1 , and Pax4 .