Exploration of Immunology
Diagnostic performance and cutoff optimisation of the CTD Screen assay for ANA detection compared with HEp-2 IFA
By Abhilash Krishnan, Yashwant Kumar, Varun Dhir and 4 others.
Abstract
Aim
Antinuclear antibodies (ANAs) are important in diagnosing systemic autoimmune rheumatic diseases (SARDs), and the HEp-2 cell indirect immunofluorescence assay (HEp-2 IFA) remains the gold standard for their detection. This study evaluates the utility and optimal cutoff of the EliA connective tissue disease Screen (CTD Screen), an enzyme immunoassay, as a complementary automated method for ANA detection.
