Hyperspectral Imaging for Objective Assessment of Skin Prick Test Reactions: A Single-Subject Proof-of-Concept Study of Interobserver Reliability and Spectral Index Separation

Background/Objectives: Hyperspectral imaging (HSI) enables non-contact assessment of tissue oxygenation and perfusion-related properties and may provide objective information beyond conventional visual assessment of skin prick test (SPT) reactions. This proof-of-concept study evaluated the interobserver reliability and within-subject spectral separation of HSI-derived indices during SPT.