Cells
Disrupting Mitochondrial Homeostasis via TBC1D15 Protein
By Pengfei Qiang, Yan Li, Shuang Liang and 3 others.
Abstract
Background
Mitochondria–lysosome coupling and mitophagy are fundamental to mitochondrial homeostasis, yet the determinants governing this process remain incompletely defined. This study distinguished the regulatory modes of TBC1D15 and established its functional role as a downstream effector in mitochondria-linked autophagy.
Methods
PC-12 and HeLa cells were treated with CCCP, gradient-concentrated AuNPs, and CQ, combined with siRNA-mediated PINK1 knockdown. Whole-cell, mitochondrial and lysosomal fractions were analyzed by immunoblotting.
