Biomolecules
Strain-Dependent Cross-Neutralization of Closely Related Asian-Lineage Zika Viruses by Dengue-Immune Sera
By Jia-Yi Tan, Nurhafiza Zainal, Sazaly AbuBakar and 1 others.
Abstract
Background
Zika virus (ZIKV) and dengue virus (DENV) co-circulate in tropical regions and elicit cross-reactive immunity. However, differential neutralization of closely related ZIKV strains remains unclear. This study characterizes the neutralization profiles of dengue-immune sera against DENV and two Asian-lineage ZIKV strains, P6-740 and PRVABC59.
Methods
Sera from suspected dengue infections and from healthy donors were screened for anti-DENV IgM and IgG antibodies and anti-ZIKV IgM and IgG antibodies by ELISA and grouped. Neutralization against DENV serotypes and two ZIKV strains was quantified using the foci reduction neutralization test (FRNT). Complete genome sequences of ZIKV P6-740 and PRVABC59 were compared to identify amino acid differences.
