Biomolecules
Template-Choice Bias in Deep Sequencing of Antibody Phage Display Libraries
By Hyunji Yang, Hwa Kyoung Lee, Taehoon Ryu and 2 others.
Abstract
Background/Objectives: Deep sequencing of antibody phage-display selections commonly uses intracellular double-stranded phagemid DNA (dsDNA), although selected libraries are propagated as virions containing single-stranded phagemid DNA (ssDNA). Whether these two templates yield equivalent quantitative profiles remains uncertain.
Methods
We sequenced matched intracellular dsDNA and virion ssDNA from a single-chain variable fragment library at the input and after each of four rounds of bio-panning.
