Evaluation of Coagulation in Rat Whole Blood, Plasma, and Serum Treated with No-Ozone Cold Plasma

Plasma has recently attracted attention in the medical field for its safety and effectiveness. Cold plasma has sterilizing, anti-inflammatory, and cancer cell-killing effects. This study investigated the coagulation effects of no-ozone cold plasma (NCP) on rat blood. The coagulation effects of NCP-treated samples at various time points were examined, along with the corresponding changes in different coagulation-related factors. The hemolysis ratio decreased in the 3 min and 5 min NCP treatment groups compared to the untreated group. Visual observation revealed clot formation, particularly at 5 min. Coagulation time results showed that prothrombin time remained unchanged, whereas the activated partial thromboplastin time was shortened in the 3 min NCP-treated group. Calcium concentrations increased in a treatment-dependent manner in the NCP-treated group. In the ELISA results, only fibrinogen showed a statistically significant increase in concentration following 5 min of NCP treatment, whereas factor XII and TGF-β showed statistically significant increases in concentration after both 3 and 5 min of NCP treatment. NCP-induced coagulation may involve the intrinsic pathway. Thus, NCP may promote blood coagulation under ex vivo conditions. However, this study suggests NCP-associated changes in coagulation parameters, rather than elucidating the underlying mechanism.