Multi-omics analysis reveals mechanisms underlying the polled phenotype in goat

In this study, transcriptome sequencing was performed on horn bud tissues of Wanling White goats at two critical developmental stages: embryonic day 70 (E70d) and postnatal day 7 (B7d). At each time point, biological replicates were strictly established, with three phenotypically confirmed horned goats (HG) and three polled goats (PG) selected, resulting in a total of 12 independent samples. This sequencing strategy was designed to systematically characterize the dynamic changes in gene expression profiles underlying horn bud development and the formation of the polled phenotype.