Abstract / Summary
Abstract It is an excellent polymer because of its high capping and stabilizing, biocompatibility, biodegradability, environmental friendliness, poly cationic, and low toxicity that has been widely utilized as a support in the synthesis of metallic nanoparticles (MNPs). The nanoparticles made of metallopolymeric chitosans may be prepared through different chemical methods, such as sol-gel, co-precipitation, and hydrothermal procedures, each possessing its own strengths of regulating particle size, shape and homogeneity. The current research used the solution-casting method to produce copper-chitosan nanoparticles (CS-CuNPs). These particles were then systematically investigated in order to test their physicochemical and biological characteristics. The produced CS-CuNPs were characterized by a stable brick-red color and a clear UV-visible absorption peak at 214 nm, which are evidence of the creation of nanomaterials and the availability of surface plasmon resonance.The X-ray diffraction (XRD) studies revealed a crystalline face-centered cubic (fcc) structure that has big diffraction peaks at 36.78°, 43.38°, 50.56°, and 74.26°. The particle size based on the atomic force microscopy (AFM) was 62.45 nm on average. Fourier transform infrared (FTIR) spectroscopy revealed the existence of strong interactions between chitosan functional groups and copper ions with distinctive absorption bands at 3370 − 3226 cm − 1 , 1633 cm − 1 , and 680 cm − 1 . Further testing was done by measuring the zeta potential (ZP) and SEM analysis to determine the stability of the colloidal particle and surface integrity of the cell lines studied and revealed that when the biological evaluation was taken into account, CS-CuNPs had much anticancer efficacy. Conversely, the cytotoxic responses of the pancreatic cancer cells were a little less encouraging, which indicated that the efficiency of nanotechnology depends on the cell line. The Cs-CuNPs showed high cytotoxic and anticancer activities at a concentration of 25 µL/mL, which portrays the potential of the new nanotherapeutic agent in cancer treatment and this is against the breast cancer cells. Graphical abstract Fig. 1.