Abstract / Summary
Abstract Bovine mastitis is a major disease affecting dairy cattle, with Staphylococcus aureus being an important etiological agent due to antimicrobial resistance and biofilm formation. This study evaluated the antimicrobial and antibiofilm activities of nisin against 25 S. aureus isolates from bovine mastitis. Antimicrobial susceptibility was assessed by disk diffusion, while nisin activity was evaluated by minimum inhibitory and bactericidal concentrations, biofilm assays, and erythrocyte hemolysis. The isolates showed high resistance to β-lactam antimicrobials. Nisin exhibited in vitro antimicrobial activity, with variable inhibitory and bactericidal concentrations among isolates. Nisin inhibited biofilm formation and slightly reduced preformed biofilms. Hemolysis was concentration-dependent, but negligible at antibacterial concentrations. These findings indicate that nisin has promising in vitro antimicrobial and antibiofilm activities against S. aureus associated with bovine mastitis, with low hemolytic activity at antibacterial concentrations, supporting its potential as an alternative approach for controlling bovine mastitis and contributing to future effective mastitis control and prevention.