Loading your page…
Investigative Ophthalmology & Visual Science
By Michelle Lin, Chen‐Ching Peng, Mark W. Reid and 10 others.
Vascular endothelial growth factor A (VEGF-A) is a key mediator of tumor angiogenesis. This cross-sectional study quantified aqueous humor (AH) VEGF-A in retinoblastoma (RB) eyes and retrospectively evaluated its association with clinical features.
Ward brings these articles into the same app as your cards and questions. One free account for Ward, Suture, Osler and Prognia.
This study analyzed 60 AH samples: 54 from 49 RB eyes and 6 from age-matched controls with congenital cataract. From pretreatment RB eyes, six samples were collected prior to globe-salvaging treatment and five immediately after primary enucleation (PE). From post-treatment eyes, 12 samples were collected at secondary enucleation (SE), 12 during active treatment (TX), and 19 at 6 months after treatment (END). VEGF-A was measured using the Olink Proximity Extension Assay and reported as absolute concentrations (pg/mL).
VEGF-A concentrations (median and interquartile range [IQR]) were 259 (95% confidence interval [CI] = 140–423, IQR = 115–568) in the RB cohort, compared with 149 (95% CI = 39.8–318, IQR = 61.3–208) in cataract controls. Post-treatment levels were significantly lower than pretreatment levels (median = 182, 95% CI = 121–310, IQR = 110–423 vs. median = 1380, 95% CI = 278–18,100, IQR = 467–12,400, P < 0.001). Among treated eyes, SE samples had higher VEGF-A than END samples (median = 506, 95% CI = 120–1010, IQR = 207–926 vs. median = 129, 95% CI = 79.5–212, IQR = 79.5–212, P = 0.007).
AH liquid biopsy was a high-yield source of VEGF-A in RB. VEGF-A concentrations were highest during primary enucleation and normalized after treatment completion. These findings support that aqueous VEGF-A reflects the intraocular microenvironment, warranting further validation to improve our understanding of its role in disease progression in RB.
Published in Investigative Ophthalmology & Visual Science. The abstract is shown as published and remains the work of its authors; read the full article at the source.
Prognia lists this article for reference and education. It is not medical advice. Always read the original and follow current guidelines and local protocols before acting on it.
