Abstract / Summary
Abstract Infected wounds remain challenging to treat because bacterial infection, biofilm formation, oxidative stress, and dysregulated inflammation impair tissue repair, while passive topical delivery limits therapeutic access to the infected wound bed. Here, we developed a nanozyme-integrated microneedle patch (MN@CuTA) by combining metal–phenolic coordination chemistry with digital light processing (DLP) 3D printing. CuTA nanozymes were incorporated into a dual-GelMA matrix. Finite element analysis optimized microneedle geometry, and DLP printing produced arrays with reliable skin penetration. Released CuTA showed dose-dependent radical-scavenging and catalase-like activities, reducing intracellular oxidative stress. MN@CuTA achieved 95.3% and 85.9% antibacterial efficiency against S. aureus and E. coli, respectively, and disrupted biofilms. MN@CuTA also promoted M2-like macrophage polarization, fibroblast migration, and endothelial tube formation. In a rat S. aureus-infected full-thickness wound model, MN@CuTA reduced bacterial burden by ∼91% on day 7 and achieved ∼96% wound closure by day 14. Thus, integrating mechanically enabled local delivery with catalytic microenvironment regulation provides an effective strategy for infected wound treatment.