Photodiagnosis and Photodynamic Therapy
Cordycepin combined with photodynamic therapy induces pyroptosis in breast cancer cells via the ROS/NLRP3/caspase-1/GSDMD signaling pathway
By Ming Zhang, Lin Zou, Ying Wang and 4 others.
Abstract
Objective
This study aimed to investigate the molecular mechanism by which cordycepin (Cor) combined with photodynamic therapy (PDT) exerts cytotoxicity against human breast cancer cells in vitro.
Methods
CCK‑8 assay, colony formation assay and Calcein‑AM/PI staining were used to evaluate cell proliferation. DCFH‑DA and JC‑1 staining combined with flow cytometry were performed to detect intracellular ROS and mitochondrial membrane potential. LDH release assay, ELISA and western blot were adopted to assess cell damage, inflammatory cytokine secretion and pyroptosis‑related protein expression.