Abstract / Summary
Background Layered plaque, representing organized thrombus overlying a previously disrupted plaque, is a marker of coronary destabilization and plaque vulnerability. Despite its clinical relevance, identifying layered plaque remains expert dependent with interobserver variability, limiting large-scale assessment. We developed and validated an artificial intelligence (AI) model for layered plaque segmentation, and assessed occurrence and associations with plaque vulnerability in non-culprit lesions. Methods Optical coherence tomography (OCT) pullbacks from the PECTUS-obs and ORANGE datasets were used to develop an enhanced version of our validated multiclass segmentation model (OCT-AID), by adding layered plaque as an additional pixel-level class. Pullbacks were divided into a training set ( n = 4121 frames, 493 with layered plaque) and an independent held-out test set ( n = 433 frames, 71 with layered plaque). Using this model, prevalence of layered plaque and associations with plaque vulnerability features were studied in patients with non-culprit lesions (PECTUS-obs data; 414 patients, 488 lesions). Results The model achieved a Dice coefficient of 0.68 ± 0.27 for layered plaque segmentation, with pullback-level sensitivity of 96.2% and specificity of 75.0%. At least one layered plaque was present in 366/414 patients (88.4%). Presence of layered plaque was higher in STEMI patients ( p = 0.018) . Lesions containing layered plaques showed higher prevalence of lipid-rich plaque (76.4% vs. 60.9%, p = 0.008 ), thin-cap fibroatheroma (29.2% vs. 15.6%, p = 0.016 ), macrophage accumulation (25.0% vs 9.4%, p = 0.010 ), and cholesterol clefts (0.0% vs. 8.3%; p = 0.009 ). Conclusions Automated OCT-based identification of layered plaque using AI is feasible. In non-culprit coronary lesions, layered plaque was highly prevalent and associated with established markers of plaque vulnerability.