Frontiers in cell and developmental biology
Serum exosomal DDX17 in the diagnosis and action mechanism of hepatocellular carcinoma
Door Huang D, Zhao M, Liu M en 7 anderen.
Inhoud in het Engels
Abstract
Background
This study aimed to explore DDX17 protein expression as a diagnostic biomarker of hepatocellular carcinoma (HCC) and its roles in the occurrence and development of HCC.
Methods
The proteomics sequencing method was applied to study nine samples of exosomes separated from HCC tissues and corresponding normal non-cancerous tissues. We also performed nanoflow cytometry analysis of DDX17 expression in serum-derived exosomes from 102 HCC patients, 58 persons with liver cirrhosis (LC), and 80 healthy donors (HDs). The feasibility of DDX17 as a diagnostic and prognostic biomarker was evaluated using receiver operating characteristic (ROC) curves. The effects of HCC-derived exosomal DDX17 on LX-2 cell proliferation, migration, and epithelial-mesenchymal transition (EMT) were evaluated through cell proliferation, colony formation, wound healing, transwell migration, and western blotting assays.