International journal of genomics
The Cellular and Immune Context of YTHDC1 in Hepatocellular Carcinoma: A Single-Cell and Multicohort Study
Door Luo S, Wen D, Zhang J en 7 anderen.
Inhoud in het Engels
Abstract
Background
YTHDC1 is a nuclear N6-methyladenosine reader involved in RNA processing. Its abundance, cellular distribution, and relationship with the immune microenvironment in hepatocellular carcinoma (HCC) remain incompletely characterized.
Methods
We integrated paired tumor-adjacent transcriptomic and proteomic cohorts, three single-cell RNA-sequencing datasets containing 249,080 quality-controlled cells, four outcome cohorts, three immune checkpoint inhibitor (ICI) cohorts, paired clinical specimens, and cell-based YTHDC1-knockdown assays. Patient-level analyses covered hepatocyte programs, immune states, expression-supported ligand-receptor axes, survival, ICI response, and exploratory single-cell network perturbation.