Prostate cancer
PROK1 Expression Is Associated With Prognosis and May Influence Response to Antiandrogen Therapy in Prostate Cancer
Door Lu DJ, Liang HQ, Li YJ en 9 anderen.
Inhoud in het Engels
Abstract
Background
Previous studies have found that PROK1 is involved in the progression of tumors. This study aims to investigate the association of PROK1 with prognosis and its potential relationship with the efficacy of antiandrogen therapy in prostate cancer and to explore the underlying mechanisms.
Methods
A total of 498 and 1249 prostate cancer samples were obtained from the TCGA and GEO databases, respectively. First, the expression of PROK1 in prostate cancer was analyzed in the bulk RNA sequencing dataset, and the prognostic value of PROK1 was explored. Subsequently, pseudotime analysis and virtual knockout were conducted in the single-cell RNA sequencing dataset to investigate its biological function in tumor progression. Further cell communication analysis revealed the interactions between cells. Additionally, the predictive function and changes of PROK1 expression in antiandrogen therapy were explored through the GDSC database and in vitro experiments.