Current Issues in Molecular Biology
Early-Apoptotic Responses of Prostate and Melanoma Cancer Cells to 6 MV X-Ray Irradiation in the Presence of Ultrasmall Gold Nanoparticles
Door Farnaz Tabatabaie, Ava Bachari en Moshi Geso.
Inhoud in het Engels
Abstract
Background
Gold nanoparticles (AuNPs) can modify cellular responses to ionizing radiation. We examined whether 1.9 nm AuNPs alter early apoptosis across a 0–8 Gy dose range in DU145 prostate and MM418-C1 melanoma cells.
Methods
Cells were incubated with or without 0.5 mM AuNPs for 24 h, irradiated with 6 MV X-rays (0, 2, 4, 6, or 8 Gy), and analysed by Annexin V-FITC/PI flow cytometry 48 h later in three independent experiments. Only adherent cells were collected for analysis; cells detached into the culture supernatant were not included. A two-factor repeated-measures ANOVA tested radiation dose, AuNP exposure, and their interaction. Because sphericity could not be assessed reliably with n = 3, a conservative Geisser–Greenhouse lower-bound correction was also applied to effects involving dose.