Anemia
Serum Metabolomic Signatures of Iron Deficiency Anemia: An Untargeted LC-HRMS-Based Case-Control Study
Par Demirel M, Köktaşoğlu F, Ağaç HD et 5 autres.
Contenu en anglais
Résumé
Objective
Iron deficiency anemia is primarily evaluated using hematological and iron-related parameters; however, these markers may not fully reflect the systemic metabolic consequences of impaired oxygen delivery and altered iron availability. This study aimed to characterize serum metabolomic alterations associated with iron deficiency anemia using an untargeted LC-HRMS approach.
Materials and methods
Serum samples from 47 individuals with iron deficiency anemia and 50 healthy controls were analyzed by LC-HRMS in positive and negative ionization modes. Data processing and metabolite annotation were performed using MassCube and MZmine workflows. Multivariate and univariate analyses, covariate-adjusted models, metabolite set enrichment analysis, correlations with hematologic/iron indices, and internally cross-validated exploratory ROC analyses were performed.