Multi-omics integration of proteomics and metabolomics in pediatric health and disease

Personalized medicine is essential for delivering efficacious care to children, yet pediatric-specific research and treatment guidelines lag behind those for adults. Multi-omics integration may offer clinicians biologically grounded patient stratification beyond traditional clinical biomarkers. While this approach holds promise for advancing precision medicine, it remains incompletely validated, and significant barriers to routine clinical implementation persist. In this review, we discuss practical considerations for integrating proteomics and metabolomics in pediatric multi-omics research. We describe popular code- and web-based tools, and recent examples of multi-omics studies in pediatric cohorts, concluding with current challenges and future outlooks for integrative multi-omics studies.