Nutrients
Exercise Is Associated with Skeletal Muscle Autophagy–Lysosome-Related Markers and Nrf2/ARF1 Signaling in HFD Mice
Par Shujuan Hu, Xuan Liu, Ruohan Xia et 1 autres.
Contenu en anglais
Résumé
Purpose
This study aimed to investigate the protective effects and potential mechanisms of exercise against long-term high-fat diet (HFD)-induced skeletal muscle dysfunction, with a focus on ADP-ribosylation factor 1 (ARF1)-related autophagy–lysosome markers and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling.
Methods
Forty male C57BL/6 mice were randomly divided into a normal diet group (DC) and an HFD group, and the latter was further subdivided into a model group (MG), exercise group (EG), Nrf2 inhibitor group (NG), and exercise combined with Nrf2 inhibitor group (ENG) after 12 weeks of HFD feeding. EG received 6 weeks of moderate-intensity treadmill exercise, NG was treated with Nrf2 inhibitor for 6 weeks, and ENG received both the 6-week treadmill exercise and inhibitor injections simultaneously. Serum lipid, blood glucose and insulin resistance indices were detected; skeletal muscle morphology was observed by H&E staining; mRNA and protein expression of ARF1, ULK1, ATP6V0A1 and other factors were determined by RT-qPCR, Western blotting and immunohistochemistry; the oxidative stress index was measured by an ELISA; and the colocalization of ARF1/ATP6V0A1 with the lysosomal marker Lamp1 was analyzed by immunofluorescence.