Journal of pain research
Acupotomy Alleviates Pain and Attenuates Spinal Neuronal Hyperexcitability in Knee Osteoarthritis Rats: Implication of the CaMKII/GluA1 Axis
Von Wu M, Teng J, Huang Z und 5 weiteren.
Inhalt auf Englisch
Abstract
Objective
To investigate the spinal mechanisms underlying the analgesic effects of acupotomy on chronic pain and central sensitization in rats with monosodium iodoacetate (MIA)-induced knee osteoarthritis (KOA).
Methods
Following MIA-induced KOA and a three-week acupotomy intervention in rats, pain behaviors and joint pathology were assessed. Spinal RNA-sequencing was performed to identify differentially expressed genes. Key targets (CaMKII and GluA1) were validated at both mRNA and protein levels using RT-qPCR and Western blotting. Furthermore, immunofluorescence and whole-cell patch-clamp electrophysiology were employed to evaluate the activation and excitability of spinal substantia gelatinosa (SG) neurons.