Frontiers in pharmacology
Spica Prunellae attenuates ER stress via GRP78/IRE1α/JNK pathway in CCL4-induced acute liver injury in mice
Von Wang Y, Hao J, Yang J und 5 weiteren.
Inhalt auf Englisch
Abstract
Objective
To evaluate the effects of Spica Prunellae (SP) on CCl4-induced acute liver injury (ALI) in mice and its regulation of the endoplasmic reticulum stress pathway.
Methods
In the toxicity test, ICR mice were administered SP decoction (2 g/kg, crude herb/mouse weight) by gavage for 10 days. Liver and kidney tissues were collected for H&E staining, and serum levels of liver function (AST, ALT) and renal (BUN, SCR) function indicators were measured. After confirming safety, 48 ICR mice were randomly assigned to normal, model, high-, medium-, and low-dose SP, and positive control groups (n = 8 per group). All mice, except the normal group, received a 10% CCl4/peanut oil intraperitoneal injection to induce ALI. The normal and model groups received saline; the positive control group received bifendate; and the SP groups received corresponding SP doses by daily gavage for 10 days. Body weight, liver, spleen, and thymus indices were recorded; ELISA measured serum ALT and AST, and liver MDA and SOD; liver histopathology was assessed by H&E staining; hepatocyte ultrastructure was examined via transmission electron microscopy; and ER stress-related proteins (GRP78, IRE1α, and JNK) expressions were determined by Western blot.